Fig. 2
From: Evolutionary loss of melanogenesis in the tunicate Molgula occulta
Expression of Tyrosinase genes during tunicate embryogenesis. The whole-mount in situ hybridization experiments show the localization of Tyrosinase gene expression in pigment cell precursors of three tunicates: Mooccu.Tyrp.a in Molgula occulta hatched larva (a), Cirobu.Tyr in Ciona robusta neurula (b), Moocul.Tyr in Molgula oculata neurula embryos (c). Double in situ hybridization of M. occulta Tyrosinase and either Neurogenin (d) or Onecut (e), showing lack of Tyrosinase expression in the developing central nervous system of embryos and larvae at various stages