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Fig. 5 | EvoDevo

Fig. 5

From: Establishment of lateral organ asymmetries in the invertebrate chordate, Ciona intestinalis

Fig. 5

Ion flux is required for proper endoderm asymmetry. a′a‴ Ventral sections of late embryonic and larval stages showing typical positioning of endodermal structures (false-colored). Cell outlines are visualized using phalloidin. b′b‴ Ventral views of Stage 31 larvae, and endodermal structures are false-colored. b′ Typical endodermal morphology in DMSO controls. b″ Typical endodermal morphology in larvae treated with the Nodal inhibitor SB431542. b‴ Typical endodermal morphology in larvae treated with the ion flux inhibitor omeprazole. c Graphical representation of data on larval endodermal asymmetry. Error bars represent SEM. n = number of larvae scored over three-independent trials and χ 2 test derived P values comparing control and omeprazole-treated larva (p = 6.9E−4) or SB431542-treated samples (p = 7.6E−7). d′e‴ Lateral sections of Ciona juveniles displaying endoderm asymmetry following DMSO (d′d‴) and omeprazole neurula stage treatment (e′e‴). Endodermal structures are false-colored. Yellow indicates symmetric endodermal organ. All scale bars are in micrometers

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