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Fig. 2 | EvoDevo

Fig. 2

From: Spatiotemporal regulation of nervous system development in the annelid Capitella teleta

Fig. 2

Ct-soxB1 is expressed in neuroectoderm and the developing CNS. a–s Ct-soxB1 transcripts were detected in stages 3–7 animals using whole-mount in situ hybridization (WMISH). The scale bar in A is 40 µm; all other images are to the same scale unless otherwise noted. Arrows in (a–d) denote expression in the anterior neuroectoderm. Black arrowhead in (h) points to expression in the pygidial ectoderm. Dashed line in (n) indicates the basal boundary of the brain lobe. Arrowheads in (o) denote Ct-soxB1 + cells in the VNC. Black arrowheads in (o) and (r) point to a circumferential row of Ct-soxB1 + cells immediately posterior to the prototroch. White arrowheads in (r) show ventrolateral rows of Ct-soxB1 + cells on either side of the developing VNC. Arrows in (r) indicate ventral ectodermal Ct-soxB1 + cells in the head. Arrow in (s) denotes expression in the PGZ. In each panel, the stage of the animal is indicated in the lower left, and the view is indicated in the lower right (lat lateral, vlat ventrolateral, vent ventral, ant anterior). In all lateral and ventrolateral views, anterior is to the left and ventral down; in all ventral views, anterior is to the left; and in all anterior views, ventral is down. Panels (f) and (j) are cropped images of the episphere from a ventral view. Panel (n) is a cropped image of the episphere from a ventrolateral view. Panel (q) is a cropped image of the episphere at 2.1× magnification. An asterisk marks the position of the mouth, and the prototroch (pt) and telotroch (tt) are labeled with dashes. In (f), (j), (n), and (q), the prototroch is denoted with dashes. ST3epi, stage 3 epiboly; STBPclg, stage 3 blastopore closing

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