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Fig. 3 | EvoDevo

Fig. 3

From: PaxA, but not PaxC, is required for cnidocyte development in the sea anemone Nematostella vectensis

Fig. 3

Distribution and development of new cnidocytes in tissues of the polyp. a Four-tentacle primary polyp illustrating the development of new cnidocytes (NCOL3 protein, red) in the ectoderm of multiple tissues. be Individual tissues from eight-tentacle-stage polyps showing expression of Ncol3 mRNA (green) in the ectodermally derived tissue (Ec) only (dotted line indicates mesoglea; En—endodermally derived tissue). The arrow in d points to a mature cnidocyte in the cnidoglandular (ectodermal) portion of the mesenterial filament. f Aggregation of mature cnidocytes encircling an ephemeral opening (asterisk) at the aboral pole of the adult polyp. The arrows in a correspond to new cnidocytes developing in the region highlighted in f. a 3D rendering from a confocal z-stack; b, c, e individual optical sections; d, f DIC micrographs

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