Fig. 3
From: PaxA, but not PaxC, is required for cnidocyte development in the sea anemone Nematostella vectensis
Distribution and development of new cnidocytes in tissues of the polyp. a Four-tentacle primary polyp illustrating the development of new cnidocytes (NCOL3 protein, red) in the ectoderm of multiple tissues. b–e Individual tissues from eight-tentacle-stage polyps showing expression of Ncol3 mRNA (green) in the ectodermally derived tissue (Ec) only (dotted line indicates mesoglea; En—endodermally derived tissue). The arrow in d points to a mature cnidocyte in the cnidoglandular (ectodermal) portion of the mesenterial filament. f Aggregation of mature cnidocytes encircling an ephemeral opening (asterisk) at the aboral pole of the adult polyp. The arrows in a correspond to new cnidocytes developing in the region highlighted in f. a 3D rendering from a confocal z-stack; b, c, e individual optical sections; d, f DIC micrographs