PaxA, but not PaxC, is required for cnidocyte development in the sea anemone Nematostella vectensis

Table 1 Morpholinos used in this study

Morpholino	Type	Target^a	Sequence (5′ → 3′)
Ctrl MO (0.9 mM)	Negative control	N/A	CCTCTTACCTCAGTTACAATTTATA
SoxB2 ATG MO (0.9 mM)	Translation-blocking	ATG	CATGCCCGTCTTCTTGCTTGCCCAT
SoxB2 5UTR MO (0.9 mM)	Translation-blocking	5′UTR	TATACTCTCCGCTGTGTCGCTA
PaxA sp MO (0.9 mM)	Splice-blocking	I1E2	AGGACCTTCAAGAACATTCGATAAT
PaxA tr MO (0.9 mM)	Translation-blocking	ATG	CCACCAGGACCTCTATGAGGCATAC
PaxC sp MO (0.6 mM)	Splice-blocking	E1I1	TCGCTCTGAATGCTTCTTACCTTCA
PaxC tr MO (0.6 mM)	Translation-blocking	ATG	GCCATAAGGAGTGGCCAGAAATCCT

^aI1E2—targets the boundary between intron 1 and exon 2 (splice acceptor site) and E1I1—targets the boundary between exon 1 and intron 1 (splice donor site). ATG—targets the start codon. 5′UTR—targets the 5′ untranslated region. All morpholinos were designed by and purchased from GeneTools, LLC (USA)

ISSN: 2041-9139