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Table 3 Primers used in this study

From: PaxA, but not PaxC, is required for cnidocyte development in the sea anemone Nematostella vectensis

Target gene

Use

Forward (5′ → 3′)

Reverse (5′ → 3′)

Ncol1

ISH

ATGGCGTTCAAGATCACACTGT

CTATTTGCAACACGCTGGGGC

Ncol1

qRT-PCR

TGGCGTTCAAGATCACACTGTTGTG

GCATGGATTAGCGTCACGTTTTTGC

Ncol3

ISH

ATGGCGTCGAAACTCATCCTCGGAGT

TTATCTTCTGCCAGCGCAGCATCCCG

Ncol3

qRT-PCR

AGATGGCGTCGAAACTCATCCTCG

TTGGCGCTGCGTTTGTATGTGC

Ncol4

ISH

ATGAGGAATAGAGTTCTCCTACTGCT

CTATTTGCCGCGACTGCAGCA

Ncol4

qRT-PCR

TCTGAAGAGAAGTCCCAACCCATGC

TGAACGAGCAACAAGCGGAAGC

Ngal

ISH

GGGGCAGTTAGGAGGCTACAGA

ACCGCTTCTCCTTGGCTGCT

Ngal

qRT-PCR

AAGCAGTACGAGCTCATCAAGG

CTGGCACTGTCCTTTGACCTTC

Ggt

ISH

CGGCATTGTGTGTAGGCGTGGT

AGGGCTTGCGGTGAGGAAGGAA

Ggt

qRT-PCR

ATACTCGTTCACCCAGTGGTTC

CATCTGTCTGTTGTGGATGCTG

Mef2IV

ISH

CACCATGGGGCGAAAGAAGATCCAG

CACATAGCTTTGCTCAGATATAG

Mef2IV

qRT-PCR

CGCATTAGCAAGACAAAGCAAGTGC

TGCAAAAAGCATTCCCCGTACTGAC

PaxA

ISH

ATGCCTCATAGAGGTCCT

GCCCTGGGTCAGTGTATCAAGG

PaxA

qRT-PCR

CAGGGAAGTCACCAATGGAGA

GCCTTTAACACTGACCAACTCG

PaxA

sp MO

ATGCCTCATAGAGGTCCT

GCCCTGGGTCAGTGTATCAAGG

PaxC

ISH

ATGGCTCACCAAATTCCATTCCAC

AACCGGAAGTCGATTGTGTCTC

PaxC

qRT-PCR

CAAGTCTCCTGGGTACGATCAC

TATCGTGCTTCGCTCTTCTGTG

PaxC

sp MO

ATGGCTCACCAAATTCCATTCCAC

AACCGGAAGTCGATTGTGTCTC

SoxB2

ISH

CGAGGCAAGAGAAAGCATTACG

ACACTCGTTGGTTACTCAGCTC

Elav a

qRT-PCR

GCGGTCTACCGAAAGACATGAA

CTACCTTCGCCGCTCACATC

RFamide a

qRT-PCR

TCGGTCGCACAATGGATACC

TCCTTAGCAGCTTGTCGCTT

Ef1B a

qRT-PCR

TGCTGCATCAGAACAGAAACCTGC

TAAGCCTTCAAGCGTTCTTGCCTG

  1. aPrimers sequences originally presented by Richards and Rentzsch [30]