Fig. 2
Fate of the 1q2 cells in the pilidium. The four middle panels show labeled domains (yellow) overlaying transmitted-light images (gray), for orientation. Peripheral panels show corresponding labeled domains alone, for clarity. Insets show enlarged or otherwise clarified views of portions of labeled domains; the color of the inset frame corresponds to the color of the outlined region of interest on the main panel. Apical organ is up on all panels. Main panels in the top row (A, D) show pilidia imaged from the left side (anterior lobe to the left); main panels in the bottom row (B, C) show pilidia imaged from the right side (anterior lobe to the right). A Contribution of the 1a2 cell: primary ciliary band, anterior left axil, a nerve. A′ The same larva (0.5× scale) as A imaged from the right side to show contribution of 1a2 to the anterior right axil. Insets to A and A′ (green and teal, respectively) show 1.8-μm-thick projections (same scale as A). Intensity values in the insets are log-transformed to make dim background visible. This highlights nuclei of axillary cells in the labeled domain because the 70 kD dextran used for labeling is excluded from nuclei. Whereas 1a2 makes the entire left axil, it only accounts for ~ 1/3 of cells in the right axil. B Contribution of the 1b2 cell: primary ciliary band, posterior right axil. Red inset shows an enlarged (2.5×) view of the axil. C Contribution of the 1c2 cell: primary ciliary band, episphere. c′ The same larva (0.5× scale) as C imaged from the left side to show contribution of 1c2 to the episphere of posterior left quadrant (arrowhead). D Contribution of the 1d2 cell: primary ciliary band, posterior left axil, episphere. Scale bar 50 μm