Fig. 5

Functional analysis by RNAi knock-down: effects of sog and dpp RNAi knock-downs in C. albipunctata. a–c Larval cuticle preparations from late-stage embryos treated with sog RNAi, exhibiting effects on dorsal closure (a), abdominal patterning (b, c), and terminal patterning (a–c, arrowheads). Panels d–i show in situ hybridisation (whole-mount, d; transverse sections, e–i). d. Blastoderm-stage embryo treated with sog RNAi stained for dpp (blue). Embryo shown in lateral view (anterior is to the left, dorsal is up). e–g Transverse sections of embryos stained for brk by in situ hybridisation. e Wild type; f embryo treated with dpp RNAi. Arrow points at dorsal de-repression of brk. Arrowhead points at the absence of brk expression around the ventral midline; g. embryo treated with gbb RNAi. h, i Transverse sections of embryos stained for twi by in situ hybridisation. h Wild type; i embryo treated with dpp RNAi