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Fig. 4 | EvoDevo

Fig. 4

From: Formation of the initial kidney and mouth opening in larval amphioxus studied with serial blockface scanning electron microscopy (SBSEM)

Fig. 4

a DIC of anterior end of a living 22-h larva focused on the left surface, showing Hatschek’s left diverticulum (asterisk) and a population of compact mesoderm cells (arrowhead) comprising the posterior region of the first left somite. Scale bar = 100 μm. b DIC of a living 23-h larva with the midsagittal plane in focus. The boxed region indicates where SBSEM series were reconstructed in 3-D in c and d. Scale bar = 100 μm. c SBSEM of 23-h larva; left side view of a 3-D reconstruction; ectoderm is not shown. The arrowhead points to a small mesodermal protuberance showing where the mouth will later form. The arrow is explained in the following figure caption. Scale bar = 10 μm. d Right side view of a 3-D reconstruction. Scale bar = 10 μm. fls, first left somite; frs, first right somite; Hld, Hatschek’s left diverticulum; Hrd, Hatschek’s right diverticulum; lpm, lateral plate mesoderm; pe, pharyngeal endoderm; sls, second left somite; srs, second right somite

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