Expression of NK cluster genes in the onychophoran Euperipatoides rowelli: implications for the evolution of NK family genes in nephrozoans

Table 3 Specific primers used to amplify the transcripts of NK homeobox genes from cDNA of the onychophoran E. rowelli

Gene	Fragment length (in bases)	Direction	Primer sequence	Annealing temperature (°C)
NK1	730	Forward	ATGTTGGTGTATTGCCGGAGG	62
NK1	730	Reverse	GTAGGCAAACTTGTGACTGGG	62
NK3	649	Forward	TCTAAGCGACACTCTCACAGC	62
NK3	649	Reverse	AGGAAGGATACATGACAGGCC	62
NK4	900	Forward	TAAAGATATTTTAAATCTTAGCGAACAAG	58
NK4	900	Reverse	GCCACATATTATTTACACAGTGACATC	58
NK5	611	Forward	CCATCGACTCAAGTTCCAACG	62
NK5	611	Reverse	ATGTTTGCTGCCTCCATCTCG	62
NK6	656	Forward	GACTACACCAACCCAAACACG	62
NK6	656	Reverse	TGGAACCATACCTTGACCTGG	62
Msx	712	Forward	TTGCAGGCGAGTAGTAACTCG	62
Msx	712	Reverse	ATGACAGTCCTAAAGCAGGCG	62
Lbx	579	Forward	CGAAGAGCATTTGAAAGCGC	58
Lbx	579	Reverse	GTCACGTGTATTCGTGTAGC	58
Tlx*	703	Forward	GCTAGAGACAATTCTCC	43
Tlx*	703	Reverse	GAACTGTCCTCAGCCC	43
NK2.2	486	Forward	ATGGCTCTAAATGGGTCAAAAAG	62
NK2.2	486	Reverse	TGGTGGATCTCCTGCATTTG	62

ISSN: 2041-9139