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Fig. 10 | EvoDevo

Fig. 10

From: Analyses of nervous system patterning genes in the tardigrade Hypsibius exemplaris illuminate the evolution of panarthropod brains

Fig. 10

Embryonic expression patterns of He-pax2/5/8. ac DIC micrographs of several specimens from He-pax2/5/8 in situ hybridizations. Arrows point to specimens that are enlarged in inset panels. Inset, a A laterally mounted embryo. The dashed line traces the ventral surface. Inset, b, c The left panels show embryos facing backward. The dashed line traces the approximate boundary of the head and first trunk segment. The right panels show embryos facing right. In the inset panel in (c), the image is mirrored. df, jj″ Confocal micrographs. He-pax2/5/8 expression is shown in green. DAPI labels nuclei (blue). d Lateral view of a 24 hpl embryo. The dashed line traces the boundary between the head and first trunk segment. The scale bar equals 10 μm. (Inset, top) The same specimen shown with DAPI signal visualized with the Cyan Hot LUT in ImageJ to help visualize morphology. The asterisks denote the position of endomesodermal pouches. (Inset, bottom) A dorsoventrally mounted 24 hpl embryo. The upper dashed line traces the boundary between the head (above) and the posterior end (below) of the embryo. An oblique coronal plane of the third (3) and fourth (4) trunk segments is shown. The lower dashed line traces the nuclei of the endomesodermal cell layer of the third and fourth trunk segments. e Lateral view of a 35 hpl embryo. Dashed lines trace the developing first and second legs. f Lateral view of a 45 hpl embryo. Dashed lines outline the inner brain region and the trunk ganglia. (Inset) The same specimen, with DAPI signal viewed with the Cyan Hot LUT in ImageJ to better visualize morphology. gi DIC micrographs of in situ hybridizations. A dashed line outlines the ventral surface of embryos. jj″ Lateral view of a 45 hpl embryo. This is the same specimen that is shown in (f). Dashed lines trace the first three legs. β-Tubulin expression (red) is shown to help visualize leg morphology. Inset, j β-tubulin expression visualized with the Glow LUT in ImageJ to help visualize the nervous system. fg, foregut; ga1–ga4, ganglion 1–ganglion 4; hpl, hours post-laying; ib, inner brain; L1–L4, leg1–leg4

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