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Fig. 6 | EvoDevo

Fig. 6

From: Analyses of nervous system patterning genes in the tardigrade Hypsibius exemplaris illuminate the evolution of panarthropod brains

Fig. 6

Embryonic expression patterns of He-otd. ac DIC micrographs of several specimens from He-otd in situ hybridizations. Arrows point to specimens that are enlarged in inset panels. a The arrowhead points to damage to the head of a specimen; this cut does not represent the body axis. (Inset ac) Specimens are facing forward in the left panels of (a, b). The solid line traces the space between the anterior and posterior ends of the specimens. In left inset panel in (c), the specimen is facing backward. The dashed line traces the boundary between the head and first trunk segment. In the right inset panels, specimens are facing right. In the right inset panel in (b), the image is mirrored. df, jj′″) Confocal micrographs. He-otd expression is shown in green. DAPI labels nuclei (blue). d Dorsal view of a 24 hpl embryo. Dashed line in the head traces presumptive developing foregut. Dashed lines also trace boundaries between the head and first trunk segment and the first (1) and second (2) trunk segments. The scale bar equals 10 μm. e Ventral view of a 35 hpl embryo. Dashed line traces the boundary between the head and first trunk segment. In the head, the dashed line traces the nuclei of the developing stomodeal complex. (Inset) The same image using the cyan hot LUT in ImageJ to aid in visualization of embryo morphology. f Lateral view of a 45 hpl embryo. Dashed lines outline the inner brain region. (Inset) The same specimen, showing a more lateral view. The region where outer brain neuropil develops is outlined. gi DIC micrographs of in situ hybridizations. A dashed line outlines the ventral surface of embryos. jj″ Face-on view of the head of a 45 hpl embryo. β-Tubulin expression is colored red. Dashed lines trace the inner brain region. j Scale bar equals 10 μm. Inset shows DAPI using the cyan hot LUT in ImageJ to aid in visualization of embryo morphology. ga1–ga4, ganglion 1–ganglion 4; hpl, hours post-laying; ib, inner brain; L1–L4, leg1–leg4; fg, foregut; mo, mouth; ob, outer brain; st, stomodeal complex

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