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Fig. 8 | EvoDevo

Fig. 8

From: Analyses of nervous system patterning genes in the tardigrade Hypsibius exemplaris illuminate the evolution of panarthropod brains

Fig. 8

Embryonic expression patterns of He-pax6 and He-unpg. ac, gi Fields of embryos at different developmental stages. a, g Arrows point to specimens that are enlarged in inset panels. Inset, a, g Anterior is toward the top. The backs of the specimens are facing forward. Dashed lines demarcate the approximate boundary between the head and first trunk segment. d, e, j, k Confocal micrographs. Gene expression is shown in green. DAPI labels nuclei (blue). af He-pax6 expression detected with in situ hybridization. d Lateral view of a 24 hpl embryo. Dashed lines trace segment boundaries. The scale bar equals 10 μm. e, f Arrows point to gene expression in the ventral trunk region. e Oblique view of a 24 hpl embryo showing expression in the head and first two trunk segments. Dashed lines trace segment boundaries and outline the developing foregut. (Inset) A deeper focal plane showing the presumptive ventral neurectoderm of all trunk segments. f Lateral view of a 24 hpl embryo. The dashed line traces the ventral surface of the embryo. gl He-unpg expression detected with in situ hybridization. j Lateral view of a 24 hpl embryo. Dashed lines trace segment boundaries. (Inset) The same specimen showing DAPI staining using the Cyan Hot LUT in ImageJ to aid in visualizing morphology. The asterisks denote the position of endomesodermal pouches. k Dorsoventral mounted 24 hpl embryo. The dashed oval in the trunk (bottom) outlines the endomesodermal cell layer of an oblique transverse section through the third trunk segment. The vertical dashed line shows the approximate position of the focal plane shown in (j). (Inset) The same specimen showing DAPI staining using the Cyan Hot LUT in ImageJ to aid in visualizing morphology. l Lateral view of a 24 hpl embryo. em, endomesodermal cell layer; fg, developing foregut; hpl, hours post-laying

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