Table 1 Ranking of candidate reference genes across eggs of haplochromine cichlid species using three different softwares

From: Maternal mRNA input of growth and stress-response-related genes in cichlids in relation to egg size and trophic specialization

BestKeeper				geNorm		NormFinder
Ranking	SD	Ranking	r	Ranking	M	Ranking	SV
NF	0.930	NF	0.805	NF	1.287	NF	0.602
mid1ip1	0.954	atf7ip	0.781	atf7ip	1.344	atf7ip	0.615
ccna1	0.969	ccnb2	0.761	mid1ip1	1.405	ccnb2	0.688
atf7ip	1.112	mid1ip1	0.679	actb1	1.511	actb1	0.688
actb1	1.286	actb1	0.678	tatdn2	1.519	xpo6	0.766
tbp	1.331	mylipa	0.635	xpo6	1.522	mid1ip1	0.785
ccnb2	1.500	rps18	0.617	ccnb2	1.522	tatdn2	0.814
rps18	1.566	tbp	0.592	gapdh	1.528	mylipa	0.815
mylipa	1.575	ccna1	0.485	ccna1	1.592	ccna1	0.900
xpo6	1.587	xpo6	–	tbp	1.634	gapdh	0.903
tatdn2	1.630	tatdn2	–	mylipa	1.653	tbp	0.911
gapdh	1.655	gapdh	–	rps18	1.752	rps18	1.028
elf1a	1.692	elf1a	–	dvr1	1.771	dvr1	1.042
rps11	1.991	rps11	–	rps11	1.943	elf1a	1.050
dvr1	2.045	dvr1	–	elf1a	2.044	rps11	1.134

SD standard deviation, r Pearson product-moment correlation coefficient, SV stability value, M M value of stability, NF normalization factor, indicating geometric mean for Cq values of atf7ip and mid1ip1 genes

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