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Table 1 Ranking of candidate reference genes across eggs of haplochromine cichlid species using three different softwares

From: Maternal mRNA input of growth and stress-response-related genes in cichlids in relation to egg size and trophic specialization

BestKeeper geNorm NormFinder
Ranking SD Ranking r Ranking M Ranking SV
NF 0.930 NF 0.805 NF 1.287 NF 0.602
mid1ip1 0.954 atf7ip 0.781 atf7ip 1.344 atf7ip 0.615
ccna1 0.969 ccnb2 0.761 mid1ip1 1.405 ccnb2 0.688
atf7ip 1.112 mid1ip1 0.679 actb1 1.511 actb1 0.688
actb1 1.286 actb1 0.678 tatdn2 1.519 xpo6 0.766
tbp 1.331 mylipa 0.635 xpo6 1.522 mid1ip1 0.785
ccnb2 1.500 rps18 0.617 ccnb2 1.522 tatdn2 0.814
rps18 1.566 tbp 0.592 gapdh 1.528 mylipa 0.815
mylipa 1.575 ccna1 0.485 ccna1 1.592 ccna1 0.900
xpo6 1.587 xpo6 tbp 1.634 gapdh 0.903
tatdn2 1.630 tatdn2 mylipa 1.653 tbp 0.911
gapdh 1.655 gapdh rps18 1.752 rps18 1.028
elf1a 1.692 elf1a dvr1 1.771 dvr1 1.042
rps11 1.991 rps11 rps11 1.943 elf1a 1.050
dvr1 2.045 dvr1 elf1a 2.044 rps11 1.134
  1. SD standard deviation, r Pearson product-moment correlation coefficient, SV stability value, M M value of stability, NF normalization factor, indicating geometric mean for Cq values of atf7ip and mid1ip1 genes