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Fig. 2 | EvoDevo

Fig. 2

From: Hox gene expression in postmetamorphic juveniles of the brachiopod Terebratalia transversa

Fig. 2

Morphology of the juvenile of Terebratalia transversa (2 days after metamorphosis), visualized with light microscopy (A) and CLSM (B, D–I). A Micrograph of the entire animal. B Frontal section through the median part of animal, cell nuclei visualized with DAPI staining. C Schematic drawing of the anatomy of juvenile in dorso-ventral (top) and lateral (bottom) views, musculature in green, tyrosinated-tubulin immunoreactive nervous system in red, acetylated-tubulin immunoreactive structures in orange (mantle margin ciliation not shown for clarity). D, G Musculature visualized with F-actin phalloidin staining, arrowheads in D point to the ventral mantle margin muscles. E, J Tyrosinated-tubulin immunoreactivity. F, I acetylated-tubulin immunoreactivity. H Transverse section through the nephrostome, cell nuclei visualized with DAPI in cyan, acetylated-tubulin immunoreactivity in yellow. A–F Dorso-ventral view, anterior to the top. G Lateral view, anterior to the left, dorsal to the top. I Virtual transverse section through the middle part of animal, dorsal to the top. J Ventral, magnified view of the commissural region from E. aam anterior shell adductor muscle, asc anterior supraesophageal commissure, ch chaeta, cr coelom rudiment, cs chaetal sac, csm chaetal sac musculature, dc dorsal commissure, dms shell diductor muscle, dt digestive tract, lr lophophore rudiment, lrf fibers in the lophophore rudiment, mc mantle cavity, mm mantle margin musculature, mmc mantle margin ciliation, mr mantle margin, nd nephroduct, np neuropil, ns nephrostome, pam posterior shell adductor muscle, pe pedicle, psc posterior supraesophageal commissure, vmc ventral mantle cavity ciliation, vpa ventral pedicle adjustor muscle

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