Fig. 3

Transverse sections through Terebratalia transversa juvenile (2 days after metamorphosis), showing detailed morphology of the animal. Each panel consists of CLSM image (left) and schematic representation (right). Cell nuclei visualized with DAPI in cyan, acetylated-tubulin immunoreactivity (F and G) and tyrosinated-tubulin immunoreactivity (E) in yellow, F-actin phalloidin staining in green. A Juvenile in dorso-ventral view, anterior to the top, dashed red lines indicates sections shown on the subsequent panels. B–H Virtual transverse sections through the juvenile, as indicated on A, dorsal to the top on all panels, on the right panels outline of the body is depicted in light blue, musculature in green, body cavities in gray, nervous system in red and excretory organs in orange. Scale bars on all images represent 20 μm. aam anterior shell adductor muscle, asc anterior supraesophageal commissure, cr coelom rudiment, csm chaetal sac muscle, dc dorsal commissure, dm dorsal mantle, dms shell diductor muscle, dt digestive tract, lr lophophore rudiment, mc mantle cavity, mm mantle margin muscle, np nephropore, ns nephrostome, pam posterior shell adductor muscle, psc posterior supraesophageal commissure, vm ventral mantle, vpa ventral pedicle adjustor muscle