Whole-mount fluorescent in situ hybridization of the Hox genes (red) combined with DAPI staining of cell nuclei (cyan, with exception of E and N) in Terebratalia transversa postmetamorphic juveniles (2 days after metamorphosis). For each plate name of the hybridized gene is provided in the right corner. Particular structures in which each of the genes is expressed are indicated with arrowheads, asterisks and double arrowheads (see text for detailed explanation). Expression of lab in chaetal sacs in dorso-ventral view (A) and virtual cross section (B); note autofluorescence of chaetae. Expression of pb in anterior shell adductor muscles in dorso-ventral view (C) and virtual parasagittal section (D). Expression of hox3 in dorso-ventral view (E). Expression of dfd in ventral pedicle adjustor muscles in dorso-ventral view (F), virtual cross section (G) and parasagittal section (H). Expression of scr in mantle margin (I). Expression of lox5 in dorso-posterior epidermis in dorso-ventral view (J) and virtual sagittal section (K). Expression of lox4 in dorso-posterior epidermis in dorso-ventral view (L) and virtual cross section (M). Expression of antp in pedicle tissue in dorso-ventral view (N, O). Expression of post2 in mantle margin, dorso-ventral view (P). Dashed lines with letters on A, C, F, J, L and O indicate section planes shown on respective plates. Scale bars on all images represent 20 μm. Anterior to the top on A, C, E, F, I, J, L, N and R; dorsal to the top on B, D, G, H, K and M; anterior to the left on D, H and K.