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Fig. 3 | EvoDevo

Fig. 3

From: Modular co-option of cardiopharyngeal genes during non-embryonic myogenesis

Fig. 3

Fluorescent in situ hybridization (FISH) showing expression of body muscle myogenic TFs during blastogenetic development of Botryllus schlosseri. a–e Double ISH showing the expression of Tbx1/10 (magenta) and Ebf (yellow). a, b FISH performed on the same animal in stage C2-D. a Magenta arrowheads point to the expression of Tbx1/10 in the dorsal mesenchyme, the dorsal region of the branchial basket, and the ventral region of the branchial basket lateral to the endostyle, and white arrowheads point to the expression of Ebf in the dorsal mesenchyme. b Posterior and transversal section of the same sample showing the expression of Tbx1/10 in the dorsal and ventral branchial basket. c Schematic representation of a developing Botryllus secondary bud in stage C2-D, showing the dorsal tube the branchial chamber, the gut, and the epidermis. d–e FISH performed in stage A1. d Magenta arrowheads point to the expression of Tbx1/10 and white arrowheads to the expression of Ebf in the mantle. O.s: oral siphon, a.s.: atrial siphon. e Magenta arrowheads point to the bilateral expression of Tbx1/10 in the intersiphonal region and white arrowheads to the expression of Ebf in the mantle and the forming cerebral ganglion. f Schematic representation of a developing Botryllus primary bud in stage A1. g Earliest expression of Ebf in few single cells of the vesicle-like secondary bud in stage B2, oriented to the primary bud and hence the future dorsal side. h Double ISH showing the expression of Ebf (yellow) and Myh3 (magenta), Ebf is expressed in mantle together with Myh3, in the cerebral ganglion and in the dorsal tube. i Schematic representation of a developing Botryllus primary bud in stage A, sagittal view. j Ebf expression in the region of the intersiphonal muscle. k Max projection showing the expression of Mrf in the region of the intersiphonal muscle. l, m Mrf expression in the mantle of the primary bud. Blue nuclei: Hoechst staining, dashed line: outline of primary bud, scale bar: 50 μm. Asterisk: unspecific staining in either tunic, gonads, or insufficiently bleached auto-fluorescent cells

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