Fig. 4
From: Modular co-option of cardiopharyngeal genes during non-embryonic myogenesis
Expression of TFs heart development-related revealed by FISH. a–c Expression of FoxF in secondary bud at stage D: a in the dorsal branchial chamber and epidermis, b in the forming heart vesicle, and c FoxF expression in the heart and epidermis in a ventral view shown by a max projection of 13 slides, stage A2. d Tbx1/10 expression in stage D. e Asymmetric Nk4 expression in stage C2, over the entire right peribranchial chamber (white arrowhead) and the right side of the branchial chamber. f Nk4 expression in a ventral view of a primary bud in stage B1. g, h Double FISH with Nk4 and Ebf in the same B2-C1 colony g in the secondary bud and h the heart of the primary bud. Nk4 restricting to the myocardium in late primary bud. Probes are indicated in the lower left corner of the pictures in the same color of the expression. Blue nuclei: Hoechst staining, dashed line: outline of primary bud, scale bar: 50 μm. Asterisk: unspecific staining in either tunic, gonads, or insufficiently bleached auto-fluorescent cells