Skip to main content
Fig. 5 | EvoDevo

Fig. 5

From: Glass confers rhabdomeric photoreceptor identity in Drosophila, but not across all metazoans

Fig. 5

glass is not expressed in rhabdomeric PRs in Platynereis. a, b glass is present in all Drosophila rhabdomeric PRs, including those in the compound eye [12, 55]. This can be observed in head cryosections, either by using in situ hybridisation (magenta in a and greyscale in a′) or with glass > mCD8::RFP flies (magenta in b and greyscale in b′). In both cases, samples were counterstained with DAPI (green). ce In contrast to Drosophila, double in situ hybridisation against the glass (red) and r-opsin1 (green) transcripts shows that glass is not present in Platynereis rhabdomeric PRs. Samples were counterstained with antibodies against acetylated Tubulin (ac-Tub, blue), which is a neuropil marker (c, transversal view of a whole-mounted, 5-day-old larva). To the right, close-ups of the dorsal (arrow in c; c′, c′′) and ventral eyes (arrowhead in c; c′′′, c′′′′) show that glass (in magenta/greyscale) is not expressed in either of these visual organs. Similarly, we found that a microinjected glass-Tomato reporter (magenta/greyscale) was not co-expressed with a stable r-opsin1-GFP insertion (green). Brightfield (BF, greyscale) was imaged as a reference (dd′′, dorsal view of a whole-mounted, 8-day-old larva). The positions of the dorsal and ventral eyes are shown with an arrow and an arrowhead, respectively. Close-ups to the right show how the axons of Tomato, and GFP-positive neurons project into two different areas in the brain (d′, d′′; orthogonal views taken along the Z segment are shown below). As a control, we also imaged an 8-day-old, wild-type, uninjected larva to test its autofluorescence (using two excitation laser wavelengths: 552 nm, same as for Tomato; and 488 nm, same as for GFP). Scale bars: 10 μm in c′, c′′′; 20 μm in de; and 50 μm in a, b. Axes: D, dorsal; M, medial; P, posterior; V, ventral

Back to article page