Fig. 11
From: Convergent evolution of saccate body shapes in nematodes through distinct developmental mechanisms
Heterodera glycines epidermis is a syncytium. a DIC (top) and fluorescent micrographs (insets) of an 8 day post-infection (DPI) J4 female following migration of epidermal nuclei and stained with both phalloidin and DAPI. At this stage, the seam cells are a single line of cells on the lateral ridge. Phalloidin fluorescence was observed surrounding each nucleus within the lateral seam, but not in the surrounding epidermis. b DIC (top) and fluorescent micrographs (insets) of an eight DPI J3 male. Phalloidin fluorescence was observed surrounding each nucleus within the lateral seam, but not in the surrounding epidermis. DAPI labels nuclei throughout the epidermis. c Fluorescent micrographs of phalloidin-stained nine DPI female following proliferation, but prior to migration of daughter epidermal cells within the lateral ridge. Scale bars, 10 µm. d TEM micrograph of nine DPI females with a cluster of nuclei on the lateral margins with distinct cell membranes. Inset, magnified image of lateral nuclei showing cell membranes (arrows) Scale bars, 200 nm