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Fig. 3 | EvoDevo

Fig. 3

From: The role of the hyaline spheres in sea cucumber metamorphosis: lipid storage via transport cells in the blastocoel

Fig. 3

Late auricularia of A. mollis with detail of hyaline spheres. a Light microscope image of 21-day auricularia showing numerous hyaline spheres (HS and *) and granular mass (GM, position shown by *) in the posterolateral larval lobes. Scale bar: 200 µm. b 19-day auricularia stained with Nile Red under blue light excitation; larva gently pressed against the glass slide using a coverslip. Hyaline spheres (HS) in all lobes stain yellow for neutral lipid. Lipid transporting cells (LTC; white circle) and granular mass (GM) stain red for a more polar lipid. Digestive system includes mouth (M), oesophagus (O), stomach (S). Scale bar: 200 µm. c Section through a hyaline sphere (HS) within blastocoel (Bl), adjacent to the ciliated band (CB) of 16-day auricularia. Macrophage-type cells (MC, white circle) are present within the HS. Arrow indicates collagen/elastin fibres inside the HS. Scale bar: 25 µm. d Hyaline sphere (HS) next to a granular mass (GM), abutting the ciliated band (CB) in one of the posterolateral larval lobes of 16-day auricularia. Arrows indicate collagen/elastin fibres in the blastocoel (Bl) and inside the HS. Scale bar: 25 µm

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