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Fig. 4 | EvoDevo

Fig. 4

From: The role of the hyaline spheres in sea cucumber metamorphosis: lipid storage via transport cells in the blastocoel

Fig. 4

Doliolaria of A. mollis. a Confocal image showing outer structure with three ciliated bands (CB) of 23-day doliolaria stained with Hoechst nuclear dye, LipidTOX™ green neutral lipid stain and LipidTOX™ red phospholipid stain. Scale bar: 50 µm. b Inner structures of same larvae as in Panel A highlighting ciliated band (CB), hyaline spheres (HS) and lipid transporting cells (LTC, white circle). Scale bar: 50 µm. c Doliolaria larva (23-day) stained with Nile Red under Nomarski light microscopy with ciliated band (CB), stomach (S), hyaline spheres (HS), granular mass (GM), area containing the 5 oral tentacles (OT, white circle), and new oral opening (Or, position shown by *). Scale bar: 100 µm. d Same larva as in Panel C under blue light excitation highlighting lipid transporting cells (LTC, white circle). Scale bar: 100 µm. e 25-day doliolaria larva with protruding oral tentacles stained with Nile Red under blue light excitation. Hyaline spheres (HS), granular mass (GM). Scale bar: 100 µm. f Section through 23-day doliolaria larva. Blastocoel (Bl); ciliary band (CB); hyaline spheres (HS) containing lipid transporting cells (white circle); oral tentacles (T); fibrillar matrix (F). Arrows indicate collagen/elastin fibres in the blastocoel pulling the HS towards the centre of the larva. Scale bar: 100 µm

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