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Fig. 2 | EvoDevo

Fig. 2

From: Novel budding mode in Polyandrocarpa zorritensis: a model for comparative studies on asexual development and whole body regeneration

Fig. 2

Asexual cycle of Polyandrocarpa zorritensis in captivity. a Polyandrocarpa zorritensis colony. Adult zooids can be found on other animals, such as mussels (yellow asterisk). Pink arrowheads show examples of individual zooids. b Bottom side of the same colony as in (a), showing the dense network of stolons and spherules. c Detail of a spherule and the stolon (red arrow) connecting the spherule to the rest of the colony. d One spherule one week after being transferred at 24 °C. The two siphons (white arrows) are open and the protrusions (red arrows) that will attach to the substrate are recognizable. e One completely developed zooid with several stolons (red arrows). e’ Close-up view of one stolon. It is possible to recognize the main blood vessel (red arrow) and the ramified ampullae connected to it (green arrows), oriented in the same direction as the main vessel. The tunic (yellow arrow) covers the whole structure. f Young budding nest. The ampullae (green arrows) increased in number along the vessel (red arrow). g Mature budding nest, composed of more compact ampullae (green arrows) that form a dome, highly pigmented on the top. h Detail of a vessel at the abscission site (white arrow). i Bud (blue arrow) developing inside the nest after abscission. j Newly budded zooid with open siphons (white arrows). k Spherule obtained by transferring a budding nest from 24 °C to 11 °C. (Scale bars ce: 1 mm; e’: 100 μm; fk: 1 mm)

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