Fig. 7

Expression of neural crest (NC) cell- and pigment progenitor markers during embryonic development and later in metamorphosis in C. azureus. a–m Whole mount in situ hybridizations (ISH), anterior to the left, dorsal view (a–l) and lateral view (m). a, b foxd3 and sox10 expression in presumptive hindbrain, mandibular- and post-otic NC streams at 36hpf. c–f, h, i foxd3 and sox10 expression in trunk NC cells migrating through the lateral- and medial routes at 42 and 48hpf. Foxd3 is also expressed in somites. g ednrb1a expression in trunk NC cells migrating through the medial route at 48hpf. h–i Higher magnifications of foxd3 and sox10 expression in trunk NC cells migrating through the lateral- and medial routes at 48hpf. j–k foxd3 and sox10 expression in trunk NC cells migrating through the lateral- and medial routes at 54hpf. Foxd3 expression is also detected in somites. l ednrb1a expression in trunk NC cells migrating through the medial route at 54hpf. m Higher magnification of ednrb1a expression in trunk NC cells migrating through the lateral route at 48hpf (somites 1–9 numbered). n–o ISH on cryosections of 11dpf/5CFRE in C. azureus larvae. Ednrb1a and mitfa expression is detected at high levels in segmented structures located dorsal to the neural tube (#), and at lower levels along myotome boundaries (arrowheads) and in the skin. e eye, hb hindbrain, lr lateral NC route, mb myotome boundary, md mandibular NC stream, mr medial NC route, my myotome, ov otic vesicle, post-otic post-otic NC stream, s somite. Scale = 250 µm