Fig. 2
From: Diverse cell junctions with unique molecular composition in tissues of a sponge (Porifera)
Immunoprecipitation results. a–c Western blot analysis of immunoprecipitation samples. Bands corresponding to the predicted molecular weight of each target protein are indicated with a red asterisk. Anti-EmITGB and Anti-EmFAK lost activity upon cross-linking to the resin, so precipitates were co-eluted with the antibody and gel slices excluding the antibody fraction were analyzed by LC–MS/MS (red boxes = gel fraction analyzed by LC–MS; blue boxes = antibody heavy chain). Equivalent gel slices were analyzed from the IgG negative control sample. a′–c′ Scatter plots showing the abundance of E. muelleri proteins detected in each precipitate relative to the IgG control. The proteins with the greatest abundance in each precipitate are indicated, with the target antigen highlighted in red. All samples were filtered to reflect only those hits within the 95% confidence interval, and represented by at least 5 unique peptides (input = whole-cell E. muelleri lysates, FT = lysate flow-through/unbound fraction, W = 1 M LiCl wash, P1-P4 = precipitate fractions 1–4)