Fig. 13

Time lapse of red and green fluorescence. Release from diapause II (A) as opposed to direct development (B). Upon release from diapause II, green cells greatly increase their number and density in less than 10 h (A). After the initial proliferation burst (c, d), green cells slowly decrease in number and density (e–f). In the lower panel, a quantification of green signal intensity as an indirect estimate of the number of green cells is reported. The lettering indicates the correspondence of the curve with the pictures (a–f). B An example of direct development. The number of green cells decreases gradually, while the number of red cells is roughly constant (a’–f’). Note that at the end of the processes the direct developed embryo (f’) is very similar to the embryo that escaped diapause (f). In the lower panel, a quantification of green signal intensity as an indirect estimate of the number of green cells is reported. The lettering indicates the correspondence of the curve with the pictures (a’–f’). Note that at the end of the processes, the ratio between red fluorescence and green fluorescence in the direct developed embryo (f’ in the right panel) is similar to that of the embryo that escaped diapause (f in the left panel)