Fig. 5

ESL nuclei as reference point for drift correction. Embryos continuously move throughout development (a–d), but EVL nuclei do not move once they reach their final position at the end of epiboly. These nuclei can therefore be used as reference system and drifts and rotations that occur during development can be corrected using their position (e–h). Correcting the drifts allows a more precise and reliable cell tracking and data analysis