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Fig. 3 | EvoDevo

Fig. 3

From: Putative stem cells in the hemolymph and in the intestinal submucosa of the solitary ascidian Styela plicata

Fig. 3

The cellular structure of Styela plicata intestine, with putative hemoblast clusters found in the submucosa. a Sagittal section of Styela plicata intestine showing its layers, clustered hemoblasts are indicated (arrowheads). b The interface of the serosa with the body cavity (coelom). Hemoblast clusters are indicated with arrowheads. Serosa projections are labeled. Asterisks mark projections of the serosa to the coelom. Note that the connective tissue contains hemoblast clusters. c Section of the mucosa, showing villi. Glandular structures can be seen beneath the epithelium (asterisks). d Section of the serosa. The asterisk marks a glandular structure. e Section of the submucosa. Hemoblast clusters are delineated (dashed line). Asterisks highlight glandular structures. f Detail of niches (delineated with dashed line) located at the outer submucosa. Arrowheads point to free cells with conspicuous granules. g Niche within the submucosa. Arrowheads point to characteristic hemoblasts. Staining techniques applied: Mallory–Cason trichrome (a, g), Hematoxylin and Eosin (ce), Gomori trichrome (b, f). (mc) mucosa, (lu) lumen of the intestine, (bm) basement membrane, (ve) villus extension, (coe) coelom. Scale bars = 5 mm in a; 2 mm in b; 50 µm in cg

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