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Table 2 Cell morphotypes used in this study to classify total hemocyte populations in Styela plicata

From: Putative stem cells in the hemolymph and in the intestinal submucosa of the solitary ascidian Styela plicata

Cell morphotype

Diameter (µm)

Percentage (%)

Possible types

Description

Small-round cells

2.8–6

16.7 ± 9.3

Lymphocyte-like cells, hemoblasts

Circular cells with a high nucleus/cytoplasm ratio. Granules scarce or absent

Granular amoeboid cells

4–8

9.9 ± 5.9

Granulocytes and amoeboid cells in differentiation

Amoeboid cytoplasm with granular contents

Large granulocytes

5–8.6

46.2 ± 9.9

Fully differentiated granulocytes, univacuolar refractile granulocytes

Rounded contour, numerous granular structures in cytoplasm, a large refractile vacuole may be present

Vacuolated cells

5–20

31.4 ± 8.8

Simple univacuolar cells, compartment cells, morula cells

Cells with one or more prominent basophilic vacuoles in the cytoplasm

Pigmented cell

8–13

3.5 ± 3.9

Pigment cell

Large cells, cytoplasm saturated with compartments filled with amber pigment