Fig. 3
Immunohistochemistry on blastula (a), gastrula (b–c) and larva stages (d–f) of Ph. harmeri. Immunohistochemistry on blastula, gastrula and larva stages labeled against acetylated tubulin (gray) and DAPI (blue). b–f Depict embryos in lateral view (lv) and b′–f′ show embryos in vegetal view (vv). In panels depicting gastrulae and larvae stages, anterior is to the left. am, anterior mesoderm; an, anus; ar, archenteron; at, apical organ; bc, blastocoel; bp, blastopore; es, esophagus; in, intestine; mo, mouth; ne, nephridium; np, nephridial primordium; pl, pre-oral lobe; pm, posterior mesoderm; st, stomach; tb, tentacle bulb; te, tentacle; tr, tentacular ridge; tt, telotroch; vp, vegetal plate. Scale bar: 25 µm