Fig. 2From: Expression of gynoecium patterning transcription factors in Aristolochia fimbriata (Aristolochiaceae) and their contribution to gynostemium developmentExpression profiles of AfimCRC, AfimSPT, AfimNGA, AfimHEC1, and AfimHEC3 genes at different floral developmental stages, and leaves using standard RT-PCR. Actin (AfimACT) was used as a positive control in the RT-PCR. S5, stage 5 of the floral bud, when stigmatic lobes are fully developed. S7, stage 7 of the floral bud, when the stigmatic lobes grow above the anthers and the ovules initiate to develop. S9, stage 9 of the floral bud, when ovules develop the two integuments. L, leaf; A, shoot apex with early floral buds (developmental stages S1–S4); Ov, ovary; Gy, gynostemium; -C indicates the amplification reaction loaded without cDNABack to article page