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Fig. 2 | EvoDevo

Fig. 2

From: Expression of gynoecium patterning transcription factors in Aristolochia fimbriata (Aristolochiaceae) and their contribution to gynostemium development

Fig. 2

Expression profiles of AfimCRC, AfimSPT, AfimNGA, AfimHEC1, and AfimHEC3 genes at different floral developmental stages, and leaves using standard RT-PCR. Actin (AfimACT) was used as a positive control in the RT-PCR. S5, stage 5 of the floral bud, when stigmatic lobes are fully developed. S7, stage 7 of the floral bud, when the stigmatic lobes grow above the anthers and the ovules initiate to develop. S9, stage 9 of the floral bud, when ovules develop the two integuments. L, leaf; A, shoot apex with early floral buds (developmental stages S1–S4); Ov, ovary; Gy, gynostemium; -C indicates the amplification reaction loaded without cDNA

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