Fig. 2
(f and g reproduced from [7] with permission from Cell Press)
Examples of trait differences between surface fish (SF) and cavefish (CF). a In situ hybridization showing differences in pax6 expression from the neural plate (top) to the late optic vesicle stages (below). ef eye field, ov optic vesicle. b In situ hybridization showing αA-crystallin expression differences in the lens viewed from lateral and dorsal sides at 40 hpf. From [53]. c Differences in adult brain morphology viewed dorsally. ot optic tectum, ol olfactory lobe. d Differences in head morphology and anti-tyrosine hydroxylase staining showing differences in olfactory neuronal regions (on) in 6 dpf larvae. e Larval skeletons showing differences in rib number (r and box). f DASPEI stained larvae showing differences in cranial neuromast density. g Assay of VAB in the laboratory showing tracings of locomotory trajectories in chambers relative to the position of a vibrating rod (center). Also see Additional file 4: Movie S4. h Differences in jaw size and tooth number in adults. te teeth. i Isolated sclera stained for cartilage (blue) and bone (red) showing differences in size and ossicle formation (os). j Differences in feeding posture behavior with respect to the substrate