Fig. 4
Crosses used in Astyanax genetics. a Cavefish × surface fish intercross showing F1 hybrid progeny with eyes and pigmentation (above) and subsequent F1 hybrid intercross showing F2 hybrid progeny with a broad range of eye sizes and 3:1 ratio of pigmented versus depigmented individuals (below). b Complementation cross between two different cavefish populations showing F1 hybrid progeny with partially restored eyes but no restoration of pigmentation. c Reciprocal (bidirectional female/male) cross showing F1 hybrid progeny with eye phenotypes suggesting maternal effects on eye degeneration