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Fig. 2 | EvoDevo

Fig. 2

From: Activin/Nodal signaling mediates dorsal–ventral axis formation before third quartet formation in embryos of the annelid Chaetopterus pergamentaceus

Fig. 2

Morphology of C. pergamentaceus. Anterior is to the left in all panels. a Adult. b DIC image in fixed L2 larva (lateral view). c Schematics depict lateral (left) and dorsal (right) views of larvae at L2 stage, approximately 36 h post-fertilization. Schematics adapted from [29]. dj are of a single L2 larva in dorsal view and ek are of a second L2 larva in lateral view. dk are merged confocal-stacked images of larvae labeled for nuclei with Hoechst (d, e), actin filaments with phalloidin (f, i), and neurons with anti-FMRFamide (j, k). Stacks of confocal micrographs of actin staining are displayed at two different depths in the body to show muscles (f, g, i) and the tripartite gut (h, i). at: apical tuft attachment point; cc: circumesophageal connective nerve; cbr: cerebral commissure; cup: cupule; dor: dorsal; mnf: modified neuropodia fans; hg: hind gut; lat: lateral; lhc: lateral hooked cilia; lo: larval ocellus; mc: main connective; mg: midgut; peri: peristomium; pp: posterior parapodia; pyg: pygidium; sc: subesophageal commissure; st: stomodeum; white arrows: cilia; white arrowheads: dorsal longitudinal muscle fibers

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