Fig. 3

In situ hybridization for smooth muscle-like effector gene expression in ACCs. aMyh9/10/11 at 10 h post-fertilization (hpf), showing expression in papilla progenitors (arrow). Expression is also strong in the notochord (noto.). bMyl9/12 at 9 hpf, showing expression in papilla progenitors (arrow) and notochord. cMyh9/10/11 expression in 17 hpf larvae, showing faint but broad signal in the papilla territory (arrow). dMyl9/12 expression in 17 hpf larvae, showing almost undetectable expression above background in papillae, a bit more in notochord. eMylk expression (green) at 17 hpf, counterstained with DAPI to label DNA (blue). Inset of boxed area shown right, indicating broad expression in the papilla territory. f Two-color in situ hybridization with Mylk (green) and CryBG (magenta) probes show strong Mylk expression in cells surrounding the protuberances, but also more weakly in the ACCs, which are labeled specifically by CryBG. Sub-cellular mRNA localization of Mylk appears quite different from that of CryBG, but still appears to localize around nuclei of ACCs (arrows). Top right: Two-color hybridization of CryBG (green) and gCalponin,hAtp2a, and iItpr (all magenta) showing ACC-specific expression. Nuclear dots (arrows) indicate likely active transcription of Itpr. All scale bars = 25 µm unless otherwise annotated