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Fig. 5 | EvoDevo

Fig. 5

From: Expression of smooth muscle-like effectors and core cardiomyocyte regulators in the contractile papillae of Ciona

Fig. 5

Intracellular calcium imaging in ACCs. a Still images captured from live time-lapse video (see Additional file 5: Video S2) of one larva electroporated with CryBG > GCamp6s, which drives expression of the calcium indicator protein GCamp6s. Transient increases in fluorescence intensity is seen in apical protrusions (arrows) around 3 s and 15 s in different cells. b Quantification of change in fluorescence in same time-lapse video, in the region of interest (ROI) 1 surrounding the apical protrusions of the ACCs (see inset), given as ΔF/F0 normalized to the maximal absolute value (1.0). See Additional file 6 for raw and normalized data

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