Fig. 6
Expression of a putative orphan G protein-coupled receptor in the ACCs. a In situ hybridization showing expression of seven-transmembrane (7TM) protein-encoding gene KH.C3.516 in the ACCs (arrows). b Two-color in situ hybridization with KH.C3.516 (magenta) and CryBG (green) shows highly specific, strong expression in the ACCs (insets). c Expression of a KH.C3.516::GFP fusion driven by the CryBG promoter shows enriched localization in the apical tips of ACC protrusions (arrows), which are exposed to the environment through the tunic. All scale bars = 25 µm