Skip to main content
Fig. 2 | EvoDevo

Fig. 2

From: Further evidence that mechanisms of host/symbiont integration are dissimilar in the maternal versus embryonic Acyrthosiphon pisum bacteriome

Fig. 2

ApNEAAT1 localization in early developing embryos prior to symbiont transmission (germarium, stages 0, 1, 5 and 6). Buchnera aphidicola is not present in the germarium, oocytes and developing embryos before stage 6. Signals representing ApNEAAT1 immunoactivity are shown in green, F-actin (Phalloidin) is in red, and nuclei (DAPI) are in blue (color key below figure). Confocal images a–c show ApNEAAT1 antibody staining and a’–c’ show merged results for ApNEAAT1 antibody, F-actin, and nuclei. Confocal images d–f are preadsorbed controls showing the antibody signal and d’–f’ are preadsorbed controls showing the merged results for ApNEAAT1 antibody, F-actin, and nuclei. g–i Illustrations of each embryonic stage. White arrowheads mark ApNEAAT1 antibody localization to the maternal follicular epithelium; yellow arrowheads mark localization to germaria membranes; arrows indicate somatic cell membrane localization. Scale bars = 10 µm. cs central syncytium, fc follicle cells, gc germ cells, gm germarium, nc nurse cells, on oocyte nucleus, oo oocyte, ps posterior syncytium, sn syncytial nucleus, st stage

Back to article page

EvoDevo

ISSN: 2041-9139