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Fig. 3 | EvoDevo

Fig. 3

From: Further evidence that mechanisms of host/symbiont integration are dissimilar in the maternal versus embryonic Acyrthosiphon pisum bacteriome

Fig. 3

ApNEAAT1 localization in embryos during symbiont transmission (stages 7, 9, and 11). Signals representing ApNEAAT1 immunoactivity are shown in green, F-actin (Phalloidin) is in red, and nuclei (DAPI) are in blue (color key below figure). Confocal images a–c show ApNEAAT1 antibody staining and a’–c’show merged results for ApNEAAT1 antibody, F-actin, and nuclei. Confocal images d–f are preadsorbed controls showing the antibody signal and d’–f’ are preadsorbed controls showing the merged results for ApNEAAT1 antibody, F-actin, and nuclei. g–i Illustrations of each embryonic stage. White arrowheads mark ApNEAAT1 antibody localization to the maternal follicular epithelium; arrows indicate somatic cell membrane localization. Scale bars = 10 µm. b endosymbiotic bacteria Buchnera, gc germ cells, hd head, st stage

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ISSN: 2041-9139