Fig. 2

3D-reconstruction images of embryos in metaphase/anaphase and in telophase. Animal-view (top) and the corresponding lateral-view (bottom) images of embryos that are double-stained for filamentous actin (green, Alexa 488-phalloidin) and β-tubulin (red, Cy3-anti-β-tubulin antibody) of the dextral embryos. Arrows indicate SD (spiral deformation). Scale bar equals 20 μm. A schematic drawing to show the formation of micromeres from respective macromeres is shown. Confocal images were obtained with a laser scanning confocal microscope (Zeiss LSM 510). 3D-reconstruction images were made from a z-series of optical sections acquired every 0.80 μm.