Fig. 3

a Time lapse of release from diapause. Images are from a double-transgenic reporter line expressing the FUCCI system under the ubiquitin promoter: red and green fluorescent cells are in G1 and S/G2/M, respectively. Time is counted from the start of the video recording. Recording starts a 0 h when most cells are in G1. In the first few hours, there is low proliferative activity (4 h), followed by a proliferation burst between 15 and 18 h (10 h and 19 h), which gradually declines (31 h). From [53] reproduced under CC. Examples of spontaneous neoplastic lesions. b liver (20Xmagnification). c cephalic kidney (10Xmagnification). d Whole-mount (WM) overview of Edu + cells in a 7-week-old Nothobranchius furzeri brain visualized 4 h after intraperitoneal injection to demonstrate the location of adult stem cell niches. Dorsal view of the entire brain. From [23] reproduced under CC. e Transferring young gut microbiome to adult fish prolongs life span. Black line control, red line homochronic (old to old) microbiome transfer late in life, purple line antibiotic treatment, and green line heterochronic (young to old) microbiome transfer late in life. Survival analysis. Statistical significance is calculated by Logrank test. * indicates a p value < 0.05; *** indicates a p value < 0.001. From [41] reproduced under CC—f Habit aridity influences evolution of lifespan in Nothobranchius. Survivorship of N. furzeri MZZW 07/01 strain (pink broken line n = 124), N. furzeri MZM 04/10 strain (red broken line, n = 113), N. furzeri MZCS 08/122 strain (brown broken line, n = 33), N. kuhntae MT-03/04 strain (light blue broken line n = 23; censored at age 33 weeks due to disease outbreak), N. kuhntae “aquarium strain” (blue broken line, n = 25), and N. kuhntae MOZ 04/07 strain (dark blue broken line, n = 24). Pooled survivorship of N. furzeri (n = 223), a species from arid habitats, is shown in solid red and the survivorship of pooled N. kuhntae (n = 72), the sister species from more humid habitats, is shown in solid blue. From [47] reproduced under CC. g Image of a transgenic tg(kif5aa:eGFP-sponge-29) f1 killifish embryo, 5 days after hatching. The transgene drives expression of GFP specifically in the brain. The green heart is driven by the cmlc2 promoter. Both promoters from zebrafish. From [42] reproduced under CC. h Image of GFP expression driven by zebrafish Ubiquitin promoter in the adult N. furzeri. From [53] reproduced under CC