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Fig. 7 | EvoDevo

Fig. 7

From: Elongation during segmentation shows axial variability, low mitotic rates, and synchronized cell cycle domains in the crustacean, Thamnocephalus platyurus

Fig. 7

EdU incorporation in anterior segments shows stereotyped progression in early Thamnocephalus larvae. a Representative larvae with three to seven segments, oriented anterior left; the trunk is posterior (right) to the gray circle (which covers the head segments for clarity). b Diagrammatic representation of larvae highlighting the progression of EdU incorporation in the trunk. a, b In each stage, the first thoracic segment (red arrowhead) and the EdU band (green asterisk) are indicated. The anterior growth zone (yellow bars) is devoid of EdU, while the posterior growth zone (blue bars) has variable numbers of cells incorporating EdU. In the last added segment, all cells incorporate EdU (green asterisk), forming a band of EdU that sometimes extends into the lateral edges of the penultimate segment. The two segments anterior to this are devoid of EdU. Anterior still, segments begin to progress through S-phase, beginning as a discretely aligned row of cells at the apical ridge of the segment that then expands throughout the segment. c, d Higher magnification of a series of hemi-segments to illustrate progression of EdU incorporation in the trunk. Thoracic segments are numbered and the EdU incorporating cells aligned along the apical ridge are indicated (arrowhead). The neuroectoderm cycles through S phase a few segments anterior to the EdU band (asterisk). Both a specimen (top) and corresponding diagrammatic representation (bottom) are given

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