Fig. 2

The effect of protein and carbohydrate concentration on the expression of IIS and TOR transcriptionally regulated genes in females and males. Surfaces show the fitted relationship between gene expression, carbohydrate level and protein level in female and male flies, based on the statistical model specified by the equation above each chart (Table 4). P = protein, C = carbohydrate, ε = error, subscripts refer to levels within each factor. Expression of (a, a′) InR, and (b, b′) 4E-BP, both negatively regulated by the activity of the IIS via the Forkhead transcription factor FOXO. c, c′ Expression of Ash2L, ostensibly negatively regulated by the activity of TOR signaling. d, d′ Expression of CG3071, ostensibly positively regulated by the activity of TOR signaling. Points indicate diets tested and dotted lines connect diets with equal protein-to-carbohydrate ratios (1:16, 1:8, 1:4, 1:2, 1:1, 2:1). Corresponding thin-plate spline plots are shown in Additional file 2: Fig. S2