Sex-specific plasticity and the nutritional geometry of insulin-signaling gene expression in Drosophila melanogaster

Table 6 Effect of including sex as an interactive versus additive term when modeling the influence of diet on the expression of individual genes

Gene	Model^a	DF^b	RSS^c	F^d	P^e
InR	T_jk = P_j + P_j²	148	100.209
	T_jk = S_k + P_j + P_j²	147	96.398	5.7500	0.0178
	T_jk = S_k (P_j + P_j²)	145	96.120	0.2098	0.811
4E-BP	T_jk = S_k + P_j	153	52.219
4E-BP	T_jk = S_k P_j	152	48.604	11.305	0.001
Ash2l	T_ik = S_k + C_i	152	80.009
	T_ik = S_k C_i	151	72.610	15.888	0.0001
	T_ik = S_k (C_i + P_j + P_j²)	147	68.459	2.229	0.069
CG3071	T_ijk = S_k + C_i + C_i² + P_j + P_j²	123	52.497
CG3071	T_ijk = S_k (C_i + C_i² + P_j + P_j²)	118	43.322	4.998	0.0003
dILP2	T_ijk = C_i + C_i² + P_j	123	157.57
	T_ijk = S_k + C_i + C_i² + P_j	122	156.47	0.8623	0.3550
	T_ijk = S_k (C_i + C_i² + P_j + C_iP_j)	119	152.96	0.9097	0.4386
dILP3	T_jk = P_j	152	136.38
	T_jk = S_k + P_j	151	134.35	2.2632	0.1346
	T_jk = S_k P_j	150	134.35	< 0.0001	0.9803
dILP5	T_ijk = C_i + P_j + P_j²	148	147.40
	T_ijk = S_k + C_i + P_j + P_j²	147	142.85	4.6873	0.0320
	T_ijk = S_k (C_i + P_j + P_j²)	144	139.80	1.0458	0.37431
dILP8	T_jk = S_k + P_j + P_j²	121	205.98
dILP8	T_jk = S_k (P_j + P_j²)	119	190.40	4.8693	0.0093

^aT is an expression level of gene, S is sex, C is carbohydrate, P is protein. The models differ by having sex as an interactive versus an additive factor. The models chosen are the simplest where one or either sex have significant parameter values
^bDegrees of freedom for each model
^cResidual sum square
^dF-statistic is against model specified in row above
^eSignificant P-values are shown in bold

ISSN: 2041-9139