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Fig. 7 | EvoDevo

Fig. 7

From: Early embryogenesis and organogenesis in the annelid Owenia fusiformis

Fig. 7

Tubulin+ and FMRFamide+ elements during neurogenesis. CLSM images of early larval stages from a 13 hpf to h 24 hpf. ad Close ups of the apical tuft innervation into the apical organ (green open arrowhead). e By 18 hpf an axon connects the apical organ with the prototroch (green closed arrowhead). f, g The apical organ connects with the neurons along the prototroch (magenta arrows), which start to be FMRFamide immunoreactive by 22–24 hpf (see Fig. 6m, n). These neurons eventually form an FMRFamide+ prototrochal ring (magenta arrowheads) which connects with the apical organ via an FMRFamide+ axon (magenta arrowheads). g, h The refringent globules that develop from 11 hpf, by 24 hpf are both FMRFamide+ and serotonin+. ao: apical organ; at: apical tuft; pt: prototroch. Scale is 25 µm in (ad). Scale bar in (eh) is 50 µm

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