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Fig. 5 | EvoDevo

Fig. 5

From: Cell-specific expression and individual function of prohormone convertase PC1/3 in Tribolium larval growth highlights major evolutionary changes between beetle and fly neuroendocrine systems

Fig. 5

Reduced viability following RNAi targeting PC1/3 and PC2. A Survival curves over 41 days after pupal injections, showing percentage of living beetles at the given time. Control (dsRed-dsRNA) n = 30, PC2 and PC1/3 n = 100. Note that all beetles had hatched on day 3 after injection and that deaths occurring before were likely caused by the injection process itself. B, C Appearance of PC2- and PC1/3-dsRNA injected pupae found dead 5 days post-injection (dpi) (lateral views). Abdomina are thin and dried out, wings are not fully elongated and not closed dorsally indicating incomplete metamorphosis. D, E Appearance of PC2- and PC1/3-dsRNA injected beetles found dead 15 days post-injection (dpi) (D: lateral view, E: dorsal view). Wings are fully elongated, but elytra were found fully (D) or half open (E). Abdomina were very flat and dry. Both appearances were found in either knockdown, see Table 2 for frequencies

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EvoDevo

ISSN: 2041-9139