Fig. 3

Locomotor and sensory cilia in the P. dumerilii larva. a SEM micrograph of a larva at 3 days post-fertilization (dpf) (nectochaete stage). Ciliary bands in the head, trunk and tail (pygidium) propel the larva in the water. Dorsal view. b A 3 dpf larva engages in swimming, as visualized by the movement of microbeads added to the water. c Bright-field image of a 3 dpf larva showing the location of adult and larval eyes. Dorsal view. d Transmission electron microscopy image of ciliary photoreceptor cells located in the larval brain. The sensory cilia (outlined in blue) of one of the two pairs of cells is shown. e Mechanosensory ciliated cells (msCells) in the pygidium labeled using a promoter construct. Acetylated tubulin labels sensory cilia (s.cilia). f EM volume reconstruction of the neurosecretory cells in the apical organ region of a 3 dpf larva. Ae: adult eye; le: larval eye; ans: apical nervous system; cPRCs: ciliary photoreceptor cells. Image in a by J. Berger; image in d by R. Shahidi