Fig. 7

Expression of Wnt4 genes. Expression of Wnt4.1 in Parasteatoda (A–C), Pholcus (D–G), Acanthoscurria (H–L (Wnt4.1), M, N (Wnt4.2)) and Phalangium (O–R). In all panels, anterior is to the left. Panels A, D, G, O and P present lateral views. Panels B, E, H, J–M, and Q show ventral views. Panel C presents a dorsal view. Panels F and R show anterior views, and panels N shows a posterior view. Panel L shows magnification of the opisthosomal limb buds (same embryo as panel K). Panels C´, H´, L´ and O´ show SYBR-green staining of the embryo shown in corresponding bright field panels. Developmental stages are indicated. The arrow in panel C points to a dorsal stripe of expression that separates pro- and opisthosoma. The arrows in panel I point to weak and small domains in the pre-cheliceral region. The arrow in panel Q points to expression in the ventral region of the opisthosoma. Expression patterns are summarized in panel S, anterior is up. Abbreviations as in Fig. 4