Fig. 10

Loser bud resorption reversed at late stage of separation from winner bud. A Post-surgery darkfield image of vasculature and two secondary buds (white dashed circles). B Both secondary buds developed to a proportional size by day four post-surgery. C Blood vessels between zooids were severed 24 h prior to imaging. Loser secondary bud showed heavy pigmentation and was greatly reduced in size. D Five days after separation, the loser secondary bud had increased in size and opened a siphon. dps days post-surgery. Scale bars = 0.5 mm